Part of the Santedame estate is also a small Capella di Santedame ("Chapel of Santedame"),  resting in the midst of the woods. It dates back to the fifteenth century and is the only remaining vestige of a twelveth-century Benedictine monastery.

The chapel was restored in the 19th century, but it is not the building in itself as much as the magnificent fifteenth-century fresco it guards, which is the real Santedame treasure. The fresco is said to belong to the Ghirlandaio school and it depicts the Virgin Mary with Saint John and Saint Peter.

The Santedame Estate also features an antique Romitorio, from the Latin romitorium, meaning "hermitage" or "solitary refuge." Santedame's Romitorio was a refuge for pilgrims travelling from the Maremma region's seaside provinces to the town of Camaldoli (the mother house for an order of Benedictine monks).